How to Calibrate your HPLC - Agilent 1220 Autosampler

How to Calibrate your HPLC - Agilent 1220 Autosampler

Calibrating the HPLC

Purpose

This document details how to properly calibrate an Agilent 1220 Autosampler HPLC to deliver accurate results for cannabis potency testing.

Responsibility

It is the responsibility of the operator to ensure that the calibrant kit being used is not expired and has been properly stored in a freezer at or below -20 °C.

Materials and Equipment 

Materials:

Calibrant Kit (Includes 100 uL of sample - estimated 4 to 6 injections per vial) 
  1. 1 ug/mL Standard (A Labeled Amber Vial)
  2. 5 ug/mL Standard (B Labeled Amber Vial)
  3. 10 ug/mL Standard (C Labeled Amber Vial)
  4. 25 ug/mL Standard (D Labeled Amber Vial) 50 ug/mL Standard (E Labeled Amber Vial)

Equipment:

  1. Agilent 1220 Autosampler HPLC system

Safety Issues

      Safety glasses and disposable nitrile gloves should always be worn when handling chemicals. In the event of skin contact, eye contact, ingestion, or inhalation, refer to the appropriate MSDS for first aid measures.

Procedure

  1. Remove the calibration standards from the -20 °C freezer and let them equilibrate at room temperature for 3 minutes.
  2. Make sure the HPLC is on and warmed up so that it is ready to run samples. a. Run through the Startup Method if the HPLC is not ready to run samples.
  3. At the Cann-ID home page, click the blue "Calibrate" button.

  1. Access the sample queue. You will notice that there are vials labeled A-E in your sample queue. These indicate that your calibration vials will be added to the sample queue to be run.
  1. Prior to placing calibrant vials into the sample tray, ensure each vial is well homogenized by vortexing them individually for 10 seconds.
  2. Remove the sample tray from HPLC and place respectively vials A, B, C, D, and E in the designated position exactly as described in the diagram above.

  1. Go back to the sample queue and click “Start” to begin the calibration. Your machine will now begin the calibration process. 
  2. Upon completion, operators must contact the Cann-ID Support to update their calibration table and update their method.

    • Related Articles

    • How to Calibrate your HPLC - Agilent 1220 Manual Injector

      Calibrating the HPLC Purpose ​This document details how to properly calibrate an Agilent 1220 Manual Injection HPLC to deliver accurate results for cannabis potency testing. Responsibility It is the responsibility of the client to ensure that the ...
    • How to Change Mobile Phase Bottles on an HPLC

      Changing Mobile Phase Bottles  Purpose The purpose of this procedure is to correctly change mobile phase bottles on an HPLC. Scope This procedure applies to HPLC instruments used by Ionization Labs and its clients. Responsibility It is the ...
    • How to Operate & Run Samples on Your Manual Injector HPLC

      For instructions on how to warm up, shut down, and run samples on your HPLC, please read the attached PDF.
    • How to Prepare Gummy Samples for HPLC Analysis

      Gummy Cannabinoid Extraction Utilizing the Quecher's Method Materials  50mL Centrifuge Tube 10mL Pipette Tips 0.5g Sodium Chloride - NaCL 2.0g Magnesium Sulfate Anhydrous MgSO4 10mLHPLC Grade Acetonitrile 10mL HPLC Grade Water 1g-1.5g Gummy ...
    • How to Prepare Flower and Oil Samples for HPLC Analysis

      Ionization Labs - Flower & Oil Sample Preparation  Purpose This procedure details how to properly prepare HPLC samples from cannabis flower material and oil material. Scope This applies to all flower and oil samples prepared at the Ionization Labs ...